Manuscript Number : SHISRRJ120329

Protective effect of Cumin on Sodium Arsenite Induced Toxicity in Swiss Albino Mice

Authors(1) :-Dr. Pramod Shanker

Arsenic in the present times has caused serious health hazards in the human population. In India, the arsenic poisoning in ground water in Gangetic plains has increased many folds. The exposed populations are suffering from various arsenicosis symptoms such as hyperkeratosis, melanosis, and other internal organ diseases of gastrointestinal, cardiovascular, hormonal and others. Hence, the present study is focused to combat the deleterious effect of arsenic toxicity in animal models utilizing medicinal plant extract. The animals (Swiss albino mice) were treated with Sodium arsenite at the dose of 8 mg per kg body weight for 16 weeks to make arsenic model and upon these arsenic pre-treated mice seed extract of Cumin at the dose of 25 mg per kg body weight was administered for 4 weeks to study the ameliorative effects of this plant extract. After the entire treatment, mice were sacrificed and their blood samples were obtained and analysed for haematological and biochemical study. The study shows that arsenic induced toxicity caused deleterious effect on the mice at the haematological and biochemical levels and there was significant normalisation in the animal at all the respective levels. Hence, Cumin possesses ameliorative properties against arsenic induced toxicity and can be used for human purpose after dose titration.

Authors and Affiliations

Dr. Pramod Shanker
TGT (Science), S.R.P.S.Govt.Sr.Sec. School, Gardanibagh, Patna, India

Sodium arsenite, Cumin, protective effect, Swiss albino miceramework, Edge Detection.

  1. D. Andrew AS, Burgess JL, Meza MM, Demidenko E, Waugh MG, Hamilton JW, Karagas MR. 2006. Arsenic exposure is associated with decreased DNA repair in vitro and in individuals exposed to drinking water arsenic. Environ Health Perspect. Aug;114(8):1193-8.
  2. Bennett MA, Huang TN, Matheson TW, Smith AK, Nickerson W. (η6- Hexamethylbenzene) ruthenium complexes. Inorganic Syntheses 1982; 21: 74–78. 58.
  3. Bones RW and Tausky HH. 1945. Colorimetric determination of creatinine by the Jaffe reaction.
  4. Biol. Chem. 158:581-591
  5. Carter DE, et al. 2003. The metabolism of inorganic arsenic oxides, gallium arsenide, and arsine: a toxicochemical review. Toxicol Appl Pharmacol.;193:309–34.
  6. Chen Y, Parvez F, Liu M, et al. 2011. Association between arsenic exposure from drinking water and proteinuria: results from the Health Effects of Arsenic Longitudinal Study. Int J Epidemiol.:828–35.
  7. Cohen SM, Arnold LL, Eldan M, Lewis AS, Beck BD. 2006. Methylated arsenicals: the implications of metabolism and carcinogenicity studies in rodents to human risk assessment. Crit. Rev. Toxicol., 36: 99–133.
  8. Draper HH, Hadley M. 1990. Malondialdehyde determination as index of lipid peroxidation.
  9. Methods Enzymol, 186: 421-431.
  10. Duker AA, Carranza EJM, Hale M 2005. Arsenic geochemistry and health. Environ. Int. 31: 631- 641.
  11. Dwivedi, N., & Flora, S. J. 2015. Sub-chronic exposure to arsenic and dichlorvos on erythrocyte antioxidant defense systems and lipid peroxidation in mice. Journal of environmental biology, 36(2), 383–391.
  12. Fawcett JK and Scott JE. 1960. A rapid and precise method for the determination of urea. J. Chem. Pathol. 13:156.
  13. Gallo M, Ferracane R, Giulia G, Ritieni A, Fagliano V. Microwave assisted extraction of phenolic compounds from four different spices. Molecules 2010; 15(3): 6366-6374.
  14. Guo S., Duan J. A., Tang Y. P., Qian Y. F., Zhao J. L., Qian D. W., Su S. L., Shang E. X., 2011.
  15. Pharmaceut. Biomed.,56(2), 264.
  16. Hajlaoui H, Mighri H, Noumi E, Snoussi M, Trabelsi N, Ksouri R. Chemical composition and biological activities of Tunisian Cuminum cyminum L. essential oil: A high effectiveness against Vibrio spp. strains. Food Chem Toxicol 2010;48: 2186-92.
  17. Hartwig A, Asmuss M, Ehleben I, Herzer U, Kostelac D, Pelzer A, Schwerdtle T, Bürkle. 2002.
  18. Interference by toxic metal ions with DNA repair processes and cell cycle control: molecular mechanisms. Environ Health Perspect.;110 Suppl 5:797-9.
  19. Helleday T, Nilsson R, Jenssen D. 2000.Arsenic [III] and heavy metal ions induce intrachromosomal homologous recombination in the hprt gene V79 Chinese hamster cells. Environ. Mol. Mutagen., 35: 114–122.
  20. Hsueh YM, Chung CJ, Shiue HS, et al. 2009. Urinary arsenic species and CKD in a Taiwanese population: a case-control study. Am J Kidney Dis. 54:859–70.
  21. Huang C, Ke Q, Costa M, Shi X. 2004. Molecular mechanisms of arsenic carcinogenesis. Mol.
  22. Cell Biochem. 255: 57-66.
  23. Jendrassik GF and Grofs BM. 1938. Quantitative colorimetric determination of bilirubin in serum or plasma. Clin.Chem. Acta. (27):79.
  24. Jha V, Garcia-Garcia G, Iseki K, et al., 2013.Chronic kidney disease: global dimension and perspectives. Lancet. 382:260–72.
  25. Johri RK. Cuminum cyminum and Carum carvi: An update. Pharmacogn Rev 2011; 5(9): 63–72.
  26. Kannan GM, Tripathi N, Dube SN and Flora SJS 2001. Toxic effects of arsenic (III) on some hematopoietic and central nervous system variables in mice and guinea pigs. Journal of Toxicology. Clinical Toxicology 39(7): 675–682.
  27. Kenneth GB, Gilbert LR. 2002. Arsenic, drinking water,and health: a position paper of the American councilon science and health. Regulatory Toxicology and Phar- macology36(2): 162–174.
  28. Kind PRH and King EJ. 1954. Determination of Alkaline Phosphatase activity in serum. J. Clin. Path. 7: 322.
  29. Kumar, P., Kumar, M., Ramanathan, A. L., & Tsujimura, M. 2010. Tracing the factors responsible for arsenic enrichment in groundwater of the middle Gangetic Plain, India: a source identification perspective. Environmental geochemistry and health, 32(2), 129–146. https://doi.org/10.1007/s10653-009-9270-5.
  30. Li R, Jiang ZT. Chemical composition of the essential oil of Cuminum cyminum L. from China.
  31. Flav Fragr J 2004; 19: 311–313.
  32. Lu M, Wang H, Li XF, Arnold LL, Cohen SM, Le XC., 2007. Binding of dimethylarsinous acid to cys-13alpha of rat hemoglobin is responsible for the retention of arsenic in rat blood. Chem Res Toxicol.;20(1):27-37.
  33. Mallikarjuna GU, Dhanalakshmi S, Raisuddin S, Rao AR. 2003. Chemomodulatory influence of Ferula assafoetida on mammary epithelial differentiation, hepatic drug metabolizing enzymes, antioxidant profiles and N-methyl-N-nitrosourea-induced mammary carcinogenesis in mice. Breast Cancer Res Treat.;81:1–10.
  34. Maurya, U. and Srivastava, S. 2011. Traditional Indian herbal medicine used as antipyretic, antiulcer, anti-diabetic and anticancer: A review. International Journal of Research in Pharmaceutical Chemistry, 4:1152-9..
  35. Miller, W.H.; Schipper, H.M.; Lee, J.S.; Waxman, S. 2002. Mechanisms of action of arsenic trioxide. Cancer Res., 62: 3893-3903.
  36. Morshedi D, Aliakbari F, Tayaranian M, Fassihi P. 2015. Cuminaldehyde as the major component of cuminum cyminum, a natural aldehyde with inhibitory effect on alpha synuclein fibrillation and cytotoxicity. Journal of Food Science: 80(10): H2336-H2345.
  37. Morshedi D, Aliakbari F. 2012. The inhibitory effects of cuminaldehyde on amyloid fibrillation and cytotoxicity of alpha-synuclein. Modares Journal of Medical Sciences: Pathobiology; 15: 45-60.
  38. NIOSH (National Institute for Occupational Safety and Health) 1994). Elements in blood or tissue. Method: 8005. In: NIOSH manual of analytical methods. 4 Edn. O.H. Cincinnati: Department of Health and Human Service, National Institute of Occupational Safety and Health. DHHS publication; 94–113.
  39. Patlolla, A.K., Tchounwou, P.B., 2005. Serum acetyl cholinesterase as a biomarker of arsenic induced neurotoxicity in Sprague-Dawley mice. International Journal of Environmental Research and Public Health, 2: 80-83.
  40. Reitman’s S. and Frankel’s S. 1957. A colorimetric method for determination of serum glutamate oxalacetic and glutamic pyruvate transaminases.” Amer J Clin Path1957; 28(1): 56-63.
  41. Saha D. 2009. Arsenic groundwater contamination in parts of middle Ganga plain, Bihar. Current Science. 97(6)753-755.
  42. Sankar, P., Telang, A. G., Kalaivanan, R., Karunakaran, V., Suresh, S., & Kesavan, M. 2016. Oral nanoparticulate curcumin combating arsenic-induced oxidative damage in kidney and brain of mice. Toxicology and industrial health, 32(3), 410–421. https://doi.org/10.1177/0748233713498455.
  43. Sekine T, Sugano M, Majid A, Fujii Y. 2007. Antifungal effects of volatile compounds from black zira (Bunium persicum) and other spices and herbs. Journal of chemical ecology; 33: 2123-32.
  44. Singh SK, Ghosh AK, Kumar A, Kislay K, Kumar C, Tiwari RR, et al. 2014. Groundwater arsenic contamination and associated health risks in Bihar, India, Int J Environ Res. 8:49‑
  45. Smith AH, Goycolea M, Haque R and Biggs ML 1998. Marked increase in bladder and lung cancer mortality in a region of northern Chile due to arsenic in drinking water. American Journal of Epidemiology 147(7): 660–669.
  46. Soni KB, Rajan A, Kuttan R. 1993. Inhibition of aflatoxin-induced liver damage in ducklings by food additives. Mycotoxin Res.;9:22–7.
  47. Styblo M, et al. 2002. The role of biomethylation in toxicity and carcinogenicity of arsenic: a research update. Environ Health Perspect.110(Suppl 5):767–71.
  48. Toro,G. et al. 1975. practical clinical chem.. P:154
  49. US NRC 2001. Arsenic in drinking water, 2001 update. Washington, DC, US National Research Council, National Academy Press.
  50. Vahidnia A, Romijn F, van der Voet GB and de Wolff F A 2008. Arsenic-induced neurotoxicity in relation to toxicokinetics: Effects on sciatic nerve proteins. ChemicoBiological Interactions 176(2-3): 188–195.
  51. Wanibuchi H, Yamamoto S, Chen H, Yoshida K, Endo G, Hori T, Fukushima S. 1996. Promoting effects of dimethylarsinic acid on N-butyl-N-(4-hydroxybutyl) nitrosamine-induced urinary bladder carcinogenesis in mice. Carcinogenesis.17:2435–2439.
  52. Wanner, J., Bail, S., Jirovetz, L., Buchbauer, G., Schmidt, E., Gochev, V., Girova, T., Atanasova, T., & Stoyanova, A. 2010. Chemical composition and antimicrobial activity of cumin oil (Cuminum cyminum, Apiaceae). Natural product communications, 5(9), 1355–1358.
  53. Yamamoto S, Konishi Y, Matsuda T, Murai T, Shibata MA, Matsui-Yuasa I, Otani S, Kuroda K, Endo G, Fukushima S. 1995. Cancer induction by an organic arsenic compound, dimethylarsinic acid (cacodylic acid), in F344/DuCrj mice after pretreatment with five carcinogens. Cancer Res.55:1271–1276.
  54. Yamanaka K, Kato K, Mizoi M, An Y, Takabayashi F, Nakano M, Hoshino M, Okada S. 2004.; The role of active arsenic species produced by metabolic reduction of dimethylarsinic acid in genotoxicity and tumorigenesis. Toxicol. Appl. Pharmacol., 198: 385–393.
  55. Zhang R. T., Chen J. H., Shi Q., Li Z. Y., Peng Z. Y., Zheng L., Wang X. R., 2014. Food Res. Int., 56(2), 47.
  56. Zheng L, Kuo CC, Fadrowski J, Agnew J, Weaver VM, Navas-Acien A. 2014. Arsenic and Chronic Kidney Disease: A Systematic Review. Curr Environ Health Rep. 1(3):192–207.
  57. Zheng LY, Umans JG, Tellez-Plaza M, et al. 2013. Urine arsenic and prevalent albuminuria: evidence from a population-based study. Am J Kidney Dis. 61:385–94.

Publication Details

Published in : Volume 1 | Issue 4 | November-December 2018
Date of Publication : 2018-11-30
License:  This work is licensed under a Creative Commons Attribution 4.0 International License.
Page(s) : 104-112
Manuscript Number : SHISRRJ120329
Publisher : Shauryam Research Institute

ISSN : 2581-6306

Cite This Article :

Dr. Pramod Shanker, "Protective effect of Cumin on Sodium Arsenite Induced Toxicity in Swiss Albino Mice", Shodhshauryam, International Scientific Refereed Research Journal (SHISRRJ), ISSN : 2581-6306, Volume 1, Issue 4, pp.104-112, November-December.2018
URL : https://shisrrj.com/SHISRRJ120329

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